Journal: International Journal of Oncology

Article Title: Doramectin inhibits glioblastoma cell survival via regulation of autophagy in vitro and in vivo

doi: 10.3892/ijo.2022.5319

Figure Lengend Snippet: Autophagy promotes the apoptosis of U87 and C6 cells. (A) DAPI staining images of U87 and C6 cells were captured using a fluorescence microscope following treatment with or without DRM (15 µ M) in the absence or presence of CQ (15 µ M) for 48h. Scale bar, 50 µ m. (B) U87 and C6 cells were treated with or without DRM (15 µ M) in the absence or presence of CQ (15 µ M) for 48 h, and the apoptotic cell ratio was measured by flow cytometry. (C) Graphical representation of quantitative analysis of the apoptotic rate. The results are presented as the mean ± SD of at least three independent experiments. ** P<0.01. CQ, chloroquine; CTR, control; DRM, doramectin.

Article Snippet: The cells were harvested, washed twice with PBS, fixed with 4% formaldehyde for 10 min at room temperature and stained with DAPI (cat. no. D8471; Merck KGaA) staining solution according to the manufacturer's instructions for 10 min at 37°C.

Techniques: Staining, Fluorescence, Microscopy, Flow Cytometry, Control